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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 3
First page : ( 1320) Last page : ( 1326)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00221.X

Isolation and Identification of Penicillium italicum from Iraqi Citrus Lemon Fruits and its Ability Manufacture of Silver Nanoparticles and their Antibacterial and Antifungal activity

Taha Zainab K.1, Howar Summaya N.1,*, Sulaiman Ghassan M.2

1Biology Department, College of Education for Pure Sciences/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq

2Biotechnology Division, Applied Science Department, University of Technology, Baghdad, Iraq

*Corresponding Author E-mail: sesame_97@yahoo.com

Online published on 18 May, 2019.

Abstract

Recently, attention to the manufacture of nanoparticles has been vital as a result to develop safe, low costeffective and environmentally friendly methods for nanoparticle manufacturing techniques. In this study, Penicillium italicum was isolated and diagnosed from Iraqi citrus lemon fruits using various media including PDAs, MEA, CDA, Nitrite sucrose (no2) Agar, CYA and YES. Isolates were cultured at different temperatures of 5, 25 and 37°C to diagnose the morphological characteristics of P. italicum, and then using P. italicum in the production of silver nanoparticles using X-Ray diffraction and Atomic Force Microscope. The antifungal and antibacterial activity was determined against Cladosporium sp, Monilia sp., Fusarium sp., Candida glabrata and Klebsiella pneumonia. The results have been shown an inhibition effect of silver nanoparticles produced by P. italicumon the growth of studies bacteria and fungi in dose-dependent manner.

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Keywords

Penicillium italicum, silver nanoparticles, Antibacterial, Antifungal.

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