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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 2
First page : ( 773) Last page : ( 786)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00136.7

Enantiomeric separation and validation of D-isomer in Pemetrexed disodium-An anti-cancer agent using Chiral HPLC

Hemchand S.1,*, Babu R. Ravi Chandra1, Annapurna Mukthinuthalapati Mathrusri2

1GITAM Institute of Science, GITAM (Deemed to be University), Visakhapatnam, India

2Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, India

*Corresponding Author E-mail: hemchand.suryadevara@gmail.com

Online published on 18 April, 2019.


Pemetrexed disodium is an anti-cancer agent. It is an anti-folate drug used for the treatment of malignant pleural mesothelioma and non-small cell lung cancer. In the present study the authors have proposed a chiral HPLC method for the separation and determination of Pemetrexed disodium and its D-isomer using Chiralpak AD-H (250 x 4.6 mm, 5 μm) column within a run time of 30 mins. A mixture of n-Hexane: Ethanol: Isopropyl alcohol: TFA (250: 650: 100: 1) was the final optimized mobile phase composition after many trials for the separation of D-isomer of Pemetrexed disodium (at 35°C) using Waters Alliance 2695 series HPLC system with 2998 photodiode array detector (UV detection at 240 nm). The method is very much useful for the pharmacokinetic studies as well as the metabolite study in vitro and in vivo for the determination of therapeutic activities of optical isomers.



Pemetrexed disodium, HPLC, D-isomer, Validation.


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