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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 2
First page : ( 625) Last page : ( 631)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00111.2

A New Stability-Indicating RP-HPLC-Pda Method for Simultaneous Estimation of Glecaprevir and Pibrentasvir in tablet dosage form

Reddy Yeragodala Narendra, Sreeramulu Jadi*, Balaswami B

Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu-515003, A.P, India

*Corresponding Author E-mail: skuynr@gmail.com

Online published on 18 April, 2019.


A simple, accurate, precise method was developed for the simultaneous estimation of the Glecaprevir and Pibrentasvir in tablet dosage form. Chromatogram was run through Std Zodiac 150 x 4.6 mm, 5μ. Mobile phase containing buffer 0.1% OPA: Acetonitrile taken in the ratio 55: 45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Glecaprevir and Pibrentasvir were found to be 2.207 min and 3.263 min. %RSD of the Glecaprevir and Pibrentasvir were and found to be 0.4 and 0.3 respectively. %Recovery was obtained as 98.90% and 99.61% for Glecaprevir and Pibrentasvir respectively. LOD, LOQ values obtained from regression equations of Glecaprevir and Pibrentasvir were 0.32, 0.97 and 0.04, 0.11 respectively. Regression equation of Glecaprevir is y= 23713.x + 11307, y = 13524x +2221 of Pibrentasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in industries.



Glecaprevir, Pibrentasvir, RP-HPLC method development, Validation.


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