Detection and Characterization of Cry1ac in BT Cotton Hybrids of MECH 162 and RCH2 Mallu Maheswara Reddy1,*, Dronavalli Nandini1, Nannapaneni Sreeja1, Kamarapu Aishwarya1, Vemula Sandeep2 1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India 2Shantha Biotechnics, Hyderabad, Telangana, India *Corresponding Author E-mail: mahesh_bt@kluniversity.in
Online published on 24 February, 2020. Abstract Background With the increase market of the transgenic crops, the demand for testing Bt cotton seeds has increased dramatically. ELISA abased protein detection and PCR based gene identification are still major approaches for detecting GMOs on large scale. Objective In our present study, PCR and ELISA based techniques was adopted to identify the specific Cry1Ac gene and proteins in MECH 162 and RCH2 transgenic cotton plants. Methods MECH 162 of 21.9ug/g and RCH2 of 17.37ug/g of seed protein was extracted. PCR analysis confirmed that gene specific CRY1Ac F/R primer as amplicon size of 550 bp for Bt gene, 600bp for nptII gene and 200bp for 35s promoter gene. Conclusion The ELISA method was used for the validation of the developed assay, also ELISA needed simple equipment and took less time. Top Keywords MECH 162, RCH2, GMO, Cry1Ac, NptII, 35s Promoter. Top |