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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 10
First page : ( 4799) Last page : ( 4802)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00829.1

Development of Analytical method by RP-HPLC Technique for determination of Secnidazole in API and Pharmaceutical Dosage Form

Rele Rajan V.*, Patil Sandip P.

Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai-400016

*Corresponding Author E-mail: drvinraj@gmail.com

Online published on 24 December, 2019.

Abstract

A novel reverse phase liquid chromatographic method protozoal, anti-amebic and antigiardiasis drug, secnidazole in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Peerless basic C18 (50mm x 4.6mm, 3μm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 80:20% (v/v). The flow rate was maintained at 1 ml/min. The detection was carried out at wavelength 310 nm. validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50–150 μg/ml for secnidazole. The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination secnidazole of in dosage form with good recoveries.

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Keywords

Secnidazole, Tri ethyl amine, Phosphoric acid, Methanol, HPLC.

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