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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 10
First page : ( 4799) Last page : ( 4802)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00829.1

Development of Analytical method by RP-HPLC Technique for determination of Secnidazole in API and Pharmaceutical Dosage Form

Rele Rajan V.*, Patil Sandip P.

Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai-400016

*Corresponding Author E-mail: drvinraj@gmail.com

Online published on 24 December, 2019.


A novel reverse phase liquid chromatographic method protozoal, anti-amebic and antigiardiasis drug, secnidazole in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Peerless basic C18 (50mm x 4.6mm, 3μm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 80:20% (v/v). The flow rate was maintained at 1 ml/min. The detection was carried out at wavelength 310 nm. validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50–150 μg/ml for secnidazole. The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination secnidazole of in dosage form with good recoveries.



Secnidazole, Tri ethyl amine, Phosphoric acid, Methanol, HPLC.


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