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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 10
First page : ( 4790) Last page : ( 4794)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00827.8

New first derivative spectrophotometric methods for the estimation of Bumetanide in tablet dosage forms

Annapurna Mukthinuthalapati Mathrusri*, Sheela Avuthu Sai, Yasaswini Rangisetty Spandana

Department of Pharmaceutical Analysis & Quality Assurance, Gandhi Institute of Technology and Management, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh-530045, India

*Corresponding Author E-mail: mannapurna.mukthinuthalapati@gitam.edu

Online published on 24 December, 2019.

Abstract

First derivative spectrophotometric methods have been developed for the assay of Bumetanide using Shimadzu UV-1800 Model double beam UV-VIS spectrophotometer in reagents such as hydrochloric acid, phosphate buffer pH 3.6, phosphate buffer pH 2.0 and distilled water. Bumetanide has shown linearity 10–70 μg/ml in all the methods and the linear regression equations are found to be y = 0.0015x -0.0013 (R² = 0.9993), y= 0.0007x 0.0004 (R² = 0.9991), y = 0.0014x -0.0015 (R² = 0.9993) and y = 0.0014x -0.0015 (R² = 0.9995) in hydrochloric acid, phosphate buffer pH 3.6, phosphate buffer pH 2.0, and distilled water respectively. The methods are validated as per ICH guidelines and found to be precise, simple and accurate.

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Keywords

First order derivative spectroscopy (D1), Bumetanide, Hydrochloric acid, Phosphate buffer, Validation, ICH guidelines.

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