Preliminary Phytochemical Screening and HPTLC Fingerprinting of Extracts of Thuja occidentalis Daharwal S. J.*, Shrivastava Suman University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh *Corresponding Author E-mail: sjdaharwal@gmail.com
Online published on 24 December, 2019. Abstract Objective To establish the fingerprint profile of Thuja occidentalis using high performance thin layer chromatography (HPTLC) technique. Methods Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3 and visionCATS software were used. Results Preliminary phytochemical screening of the extract showed the presence of alkaloids, triterpenes, tannins, saponins, glycosides, phenolic compounds and flavonoids. HPTLC finger printing of methanol extract of plant with Rf values at 0.5. Conclusions It can be concluded that HPTLC fingerprint analysis of extract of Thuja occidentalis can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker. Top Keywords Thuja occidentalis, Phytochemical Screening, HPTLC Fingerprinting. Top |