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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 1
First page : ( 331) Last page : ( 338)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00060.X

Relative Bioremediation of Used engine oil Contaminated soil from an Industrialised area by Various microbes

Sivakumar J.1, Sundaram C. Shanmuga2, Krishnasamy L.1, Rao U. S. Mahadeva3,*

1PG & Research, Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai-603 103

2PG & Research, Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai-603 103

3Faculty of Medicine, Universiti Sultan Zainal Abidin, 20400, Terengganu, Malaysia

*Corresponding Author E-mail: raousm@gmail.com

Online published on 16 March, 2019.



The present study is to degrade the engine oil through microbes from the industrialized areas in and around Chrompet, Chennai. Engine oil contaminated soil samples were collected from different locations of Chrompet which is located 35 KM away from Chennai.


As a result of spread plate technique the microbial colonies were enumerated and three different organisms such as bacteria namely Pseudomonas sp and Bacillus sp.Actinomycetesnamely Streptomyces sp and funginamely Aspergillus flavus and Aspergillus niger were identified. The bacterial colonies were identified by morphological and biochemical techniques using the taxonomic scheme. pH and optical density (growth rate) was determined for Pseudomonas sp, Bacillus sp, consortium (Pseudomonas sp and Bacillus sp), Streptomyces sp, Aspergillusflavus and Aspergillus nigerto the concentration from 1ml to 5ml for 16 days.


All the isolated microorganisms had the potential to degrade the engine oil. Comparatively the bacterial consortium had the highest potential to degrade the engine oil.


This formulation may be the best solution to solve the land pollution by engine oil and also to conserve the natural environment.



Aspergillus flavusAspergillus nigerBacillus sp, Consortium, Engine oil, Pseudomonas spStreptomyces sp.


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