Detection of Benzo[a]Pyrenediol epoxide-DNA Adducts in White Blood Cells of Asphalt Plant Workers in Syria Zohairee Razan1,*, Zamrik Mohammad Amer2, Barguil Sophie3 1Master Student DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University 2Professor DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University 3Assistant Professor DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University *Corresponding Author E-mail: rita.joo@hotmail.com
Online published on 20 December, 2018. Abstract Background Benzo[a] pyrene is considered a classic DNA-damaging carcinogen and is a lead compound of polycyclic aromatic hydrocarbons. B[a]Pis metabolically activated by cytochrome P450 enzymes to formation electrophilic metabolites such as epoxides. These epoxides might bind to DNA and produce DNA-adducts. ELISAmethod is used for determination of DNA adducts of benzo[a] pyrenediolepoxide (BPDE). Objectives The Aim of this study was to evaluate exposure to B[a]P in asphalt plant workers by measuring the BPDE-DNA adducts in their peripheral white blood cells (WBC), which are considered biological markers for exposure risk assessment. Materialand Methods In this study, the levels of BPDE-DNA adducts were measured in DNA samples of WBC obtained from asphalt plant workers in Syria and compared to those measured from a control group. The measurement was performed using BPDE-DNA Adducts ELISA.kit. The sample size was determined to be 50 with 25 asphalt plant workers and 25 healthy volunteers with no occupational exposure to Benzo[a] pyrene. Results BPDE-DNA adducts were detected in WBC of 11 asphalt plant workers with concentrations ranging between 0 and 2.75 ng/ml and only one individual in the control group with concentration of 0.75 ng/ml. These Results indicate a significant positive relationship betweenexposure toB[a]P through the bitumen fumes (working in asphalt plant) and formation of BPDE-DNA adducts. Conclusions BPDE-DNA adducts area potential biomarker for PAHs exposure and likely helpful indicator of PAH-induced DNA damage. Top Keywords Benzo[a] pyrene, BPDE-DNA adducts, WBC, Asphalt workers, Exposure. Top |