(34.239.160.86)
Users online: 2478    [ij] [ij] [ij] 
Email id
 

Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 9
First page : ( 3851) Last page : ( 3856)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00705.9

New stability indicating liquid chromatographic method for the determination of Pterostilbene in capsules

Bindu Gujju. Hima*, Annapurna Mukthinuthalapati Mathrusri

Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam-530045, India

*Corresponding Author E-mail: bindureddy.frnd@gmail.com

Online published on 20 December, 2018.

Abstract

Pterostilbene is used for the treatment of cancer, diabetes, anti-hyperlipidemic and fungal infections. A new RP-UFLC method has been developed for the determination of Pterostilbene. Shimadzu Model UFLC system, equipped with PDA detector and Sunfire C18 column was used for the present chromatographic study. Mobile phase consisting of a mixture of tetra butyl ammonium hydrogen sulphate andacetonitrile (32: 68, v/v)with flow rate 1.0 mL/min was chosen for the determination of Pterostilbene. Linearity was observed over the concentration range 0.1–100 μg/ml (R2 = 0.9999) with linear regression equation y = 161775.7385x 15849.1207. The LOD and LOQ were found to be 0.01642 μg/ml and 0.05421 μg/ml respectively. Solutions of Pterostilbenewere subjected to stress conditions such as acidic, alkaline, oxidation and thermal degradations. The method was validatedas per ICH guidelines. This method is quite suitable for the determination of Pterostilbene in pharmaceutical formulations.

Top

Keywords

UFLC, Pterostilbene, Stability-indicating, Validation, ICH guidelines.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
514,091,105 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.