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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 8
First page : ( 3633) Last page : ( 3637)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00669.8

PRIMER1: A Network Service for Tetra-Arms PCR Primer Design Based on Well-Known dbSNP

Al-Koofee Dhafer A. F.*, Mobarak Shaden M. H.

Dept. of Clinical Laboratory Science, Faculty of Pharmacy, University of Kufa, Kufa, P.O. Box (21), Najaf Governorate, Iraq

*Corresponding Author E-mail: dhafera.faisal@uokufa.edu.iq

Online published on 31 October, 2018.

Abstract

Summary

One of the most essential roles in polymerase chain reaction (PCR) primer design is a gene structure, predominantly for eukaryotic cells genes with numeral single-nucleotide polymorphisms (SNPs) which impose on users the discrimination between them via many techniques involving PCR. At this point, PRIMER1 is illustrated as suitable network server used for primer design to detect SNP by a simple and economical method named tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR). In PRIMER1, the users are able to concentrate their attention on the dbSNP NCBI and primer design delineation without wasting time identifying the positions of SNPs manually configuring difficult limitation.

Accessibility

PRIMER1 is freely accessible at: http://primer1.soton.ac.uk/primer1.html. The network server supports the browsers: Firefox, Chrome, Safari, Opera, and Torch.

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Keywords

SNP, Tetra-ARM-PCR, SBE, dbSNP.

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