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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 8
First page : ( 3427) Last page : ( 3430)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00632.7

Evaluation of Immunomodulatory activity of Ficus dalhousiae Miq leaves Methanolic extract against Cyclophosphamide Induced Myelosuppression

Ghori Syed Safiullah*, Ruqsar Ayesha, Akram Mohammed, Fatima Zehra, Arafath Mir Iftekhar Ali

Pharmacology Research Lab, Anwar-Ul-Uloom College of Pharmacy, New Mallepally, Hyderabad, 500016, Telangana, India

*Corresponding Author E-mail: safiullahghori@gmail.com

Online published on 31 October, 2018.

Abstract

The immunomodulatory activity of Ficus dalhousiae Miq leaves methanolic extract was evaluated against cyclophosphamide induced immunosuppression in rats. Methanolic extract of leaves of Ficus dalhousiae (250mg/kg and 500mg/kg p.o.) were administered for 13 days to wistar albino rats and cyclophosphamide (30mg/kg i.p.) was administered on 11th,12th and 13th day 1 hour after the administration of the respective treatment. On the 14th day blood was collected by retro orbital puncture and the activity was evaluated by determining the RBC, Hb%, Platelet, total WBC and differential leucocyte count. Methanolic extract of leaves of Ficus dalhousiae showed very significant (p<0.001) counteracting effect to cyclophosphamide induced reduction in total WBC, DLC and platelet count and significant (P<0.01) effect to that of reduction in RBC counts and Hb%. The significant immunostimulant effect of the methanolic extract of Ficus leaf on cyclophosphamide induced myelosuppression may be attributed towards the collective presence of saponins, and tannins in the extract.

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Keywords

Ficus dalhousiae Miq, cyclophosphamide, immunostimulant, differential leucocyte count (DLC), Hb (haemoglobin).

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