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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 8
First page : ( 3363) Last page : ( 3366)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00618.2

Detection of Benzo[a] pyrenediol epoxide-DNA adducts in White Blood Cells of Asphalt Plant Workers in Syria

Zohairee Razan1, Zamrik Mohammad Amer2, Barguil Sophie3

1Master Student DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University

2Professor DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University

3Assistant Professor DPT of Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University

Corresponding Author E-mail: rita.joo@hotmail.com

Online published on 31 October, 2018.

Abstract

Background

Benzo [a] pyrene is considered a classic DNA-damaging carcinogen and is a lead compound of polycyclic aromatic hydrocarbons.B [a] Pis metabolically activated by cytochrome P450 enzymes to formation electrophilic metabolites such as epoxides. These epoxides might bind to DNA and produce DNA-adducts. ELISA method is used for determination of DNA adducts of benzo [a] pyrenediolepoxide (BPDE).

Objectives

The aim of this study was to evaluate exposure to B [a] P in asphalt plant workers by measuring the BPDE-DNA adducts in their peripheral white blood cells (WBC), which are considered biological markers for exposure risk assessment.

Material and Methods

In this study, the levels of BPDE-DNA adducts were measured in DNA samples of WBC obtained from asphalt plant workers in Syria and compared to those measured from a control group. The measurement was performed using BPDE-DNA Adducts ELISA kit. The sample size was determined to be 50 with 25 asphalt plant workers and 25 healthy volunteers with no occupational exposure to Benzo [a] pyrene.

Results

BPDE-DNA adducts were detected in WBC of 11 asphalt plant workers with concentrations ranging between 0 and 2.75 ng/ml and only one individual in the control group with concentration of 0.75 ng/ml. These results indicate a significant positive relationship betweenexposure toB [a] P through the bitumen fumes (working in asphalt plant) and formation of BPDE-DNA adducts.

Conclusions

BPDE-DNA adducts area potential biomarker for PAHs exposure and likely helpful indicator of PAH-induced DNA damage.

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Keywords

Benzo [a] pyrene, BPDE-DNA adducts, WBC, Asphalt workers, Exposure.

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