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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 7
First page : ( 3049) Last page : ( 3056)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00561.9

Development and Validation of Spectrophotometric Determination of Glimepiride in Pure and Tablet Dosage forms through Ion-Pair Complex Formation using Bromothymol Blue

Ramadan Abdul Aziz*, Zeino Souad

Department of Chemistry, Faculty of Science, University of Aleppo, Syria

*Corresponding Author E-mail: dramadan@scs-net.org

Online published on 31 October, 2018.


A simple, direct and accurate spectrophotometric method has been developed for the determination of Glimepiride (GLM) in pure and pharmaceutical formulations by complex formation with bromothymol blue (BTB). The method involves the formation of a yellow ion-pair complex between BTB with glimepiride (C24H34N4O5S) at pH<3,8; after reacting GLM with Na2CO3 to give C24H33N4H+O5NaS which is extracted by chloroform. The formed complex [GLM]:[BTB] was measured at λmax 412 nm against the reagent blank prepared in the same manner. Variables were studied in order to optimize the reaction conditions. Molar absorptivity (ε) for complex was 18024 L mol−1 cm−1. Beer's law was obeyed in the concentration range of 2.453 -49.062 μg.mL−1 in present of 5.0x10−4 mol.L−1 of BTB with good correlation coefficient (R2= 0.9995). The relative standard deviation did not exceed 3.8%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.30 and 0.91 μg.mL−1, respectively. The proposed method was validated for specificity, linearity, precision and accuracy, repeatability, sensitivity (LOD and LOQ) and robustness. The developed method is applicable for the determination of GLM in pure and different dosage forms with average recovery of 99.5 to 101.6% and the results are in good agreement with those obtained by the RP-HPLC reference method.



Direct spectrophotometric method, Glimepiride, Bromothymol blue, Ion-pair complex.


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