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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 7
First page : ( 2965) Last page : ( 2970)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00547.4

Diagnosis and Distinguishing of Entamoeba spp. by PCR Technique

Al-Hassany Najim Abd Al-Wahid*, Al-Jabery Ahmed Hieran Ramadhan

Department of Biology, College of Science, University of Al-Qadisiyah, Iraq

*Corresponding Author E-mail: Sci.najim.alhssanie@yahoo.com

Online published on 31 October, 2018.

Abstract

This study conducted between October 2016 to September 2017, with the aims of distinguishing between amoeba species by molecular methods and determination of the prevalence of pathogenic and non-pathogenic species, In Imam Hussein Teaching Hospital, in the province of ThiQar southern Iraq. 96 stool samples were positive in microscopic examination. After the PCR test, positive samples were found in 86 samples and 10 negative samples E.histolytica were the most common 82 (85.4%) Followed by E.dispar14 (14.5%) These two parasites mixed infection E.histolytica+E.dispar 10 (10.4%) cases, and E.histolytica+ E. hartmanni 8 (8.3%) cases.

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Keywords

Diagnosis, DNA Sequance, Entamoeba spp, PCR technique, phylogenetic tree.

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