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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 6
First page : ( 2541) Last page : ( 2545)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00469.9

Preparative HPLC method for the isolation of compounds from Euphorbia hirta Linn. ethanol extract

Asha S.1,*, Thirunavukkarasu P.2, Sadiq A. Mohamed3,**

1Department of Biochemistry, D.K.M. College for Women (autonomous), Sainathapuram, Vellore DT, Tamil Nadu-632001

2Department of Biotechnology, Dr. M.G.R. Educational and Research University, Chennai

3PG and Research Department of Biochemistry, Adhiparasakthi College of Arts and Science (autonomous), G.B. Nagar, Kalavai, Vellore DT, Tamil Nadu-632506

*Corresponding Author E-mail: asha.sivaji@gmail.com

**mohamed68@rediffmail.com

Online published on 24 August, 2018.

Abstract

Two compounds were isolated from the leaf part of Euphorbia hirta Linn growing in Tamil Nadu, India. The compounds were isolated by preparative HPLC and their purity was evaluated by HPLC-DAD. Their structures were established on the basis of detailed spectroscopic techniques like FTIR, NMR and LCMS.

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Keywords

Euphorbia hirta, Preparative HPLC, HPLC-DAD, LC-MS, FTIR, NMR, lupeol, Quercetin.

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