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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 5
First page : ( 1867) Last page : ( 1876)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00347.5

Phytochemical analysis and isolation of endophytic bacteria from Bauhinia purpurea

Sunkar Swetha*, Akshaya A, Aarthi B, Nachiyar C. Valli, Prakash P.

Department of Biotechnology, Sathyabama University, Chennai, 119, Tamilnadu, India

*Corresponding Author E-mail: swethauk78@gmail.com

Online published on 21 August, 2018.

Abstract

The medicinal value of Bauhinia purpurea is well known in traditional medicine. This study was aimed to analyse the phytochemicals present in the extract prepared using stem, fruit and leaves. Qualitative analysis of phytochemicals showed positive result for alkaloids, saponin, phenol, flavonoids, anthocyanin, terpenoids, and steroids. The fluorescence analysis was also carried out for the extract using different solvents. Similarly, the extract was checked for its anti-bacterial and antioxidant activity. The extract showed notable biological activities which well correlates with the presence of different phytochemicals. Also, an attempt has been made to isolate endophytic bacteria from Bauhinia purpurea. Three endophytic bacterial isolates named as Bb1, Bb2 and Bb3 were isolated after surface sterilization. These bacteria were found to have appreciable antioxidant and antibacterial activity. These isolates were characterised based on biochemical parameters and 16srRNA sequencing. One isolate was identified to belong to Bacillus sp based on BLAST analysis. These isolates were found to possess substantial antioxidant and antibacterial activities. This study provides a preliminary report on the biological potential of Bauhinia purpurea and its endophytic bacteria.

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Keywords

Bauhinia purpurea, phytochemicals, TLC, GC-MS, endophytic bacteria.

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