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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 4
First page : ( 1513) Last page : ( 1515)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00281.0

The importance of diagnosing Mycobacterium tuberculosis by real time PCR Compared with the approved diagnostic methods in the Clinical Laboratory

Doya Lama1, Dalloul Danial1,*, Alkhayer Mohammad2, Yazigi Haissam3

1Postgraduate Student in Laboratory Diagnosis Department, Faculty of Medicine, Tishreen University, Latakia-Syria

2Professor at Pulmonary Diseases Department Faculty of Medicine, Tishreen University, Latakia-Syria

3Professor at Laboratory Diagnosis Department, Faculty of Medicine, Tishreen University, Latakia-Syria

*Corresponding Author E-mail: drdanialdalloul@gmail.com

Online published on 24 July, 2018.

Abstract

Background

Tuberculosis remains a serious public health problem worldwide. Zheil-Neelsen stained smear and culture on Lowenstein Jensen(LJ) media are conventional methods used for the diagnosis of Mycobacterium tuberculosis in most developing countries. PCR for the diagnosis of tuberculosis is not evaluated in developing countries.

Aim

To evaluate the ability of PCR to diagnose MTB in pulmonary and extra-pulmonary samples, and to compare it with the results of ZN and LJ.

Methods

Samples obtained from 96 patients of suspected TB (pulmonary and extra-pulmonary) were processed for detection of MTB by ZN smear examination, LJ medium culture and Real time PCR test.

Results

The sensitivity of PCR was 100% compared to 17, 4% for smear microscopy and 100% for LJ culture as a golden standard. PCR sensitivity in pulmonary and extra-pulmonary samples was 96.2% and 100% respectively. The mean detection time for MTB was 6 weeks by LJ medium culture, less than one day by PCR and 4 days by ZN for 96 samples.

Conclusions

PCR is a rapid and sensitive method for the early diagnosis of pulmonary and extra-pulmonary tuberculosis.

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Keywords

Mycobacterium Tuberculosis, Real time PCR, ZN staining, LJ medium culture, pulmonary TB, extra pulmonary.

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