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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 4
First page : ( 1313) Last page : ( 1315)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00244.5

Disinfectant Susceptibility Testing of Non-Fermenting Gram Negative Bacilli

Mrs. Bhuvaneshwari G.1,*, Dr. Shameembanu A. S.2, Dr. Kalyani M.3

1Department of Microbiology, Saveetha University, Saveetha nagar, Thandalam, Chennai, India

2Department of Microbiology, Chettinadu Medical College and Research Institute, Chennai, India. Shameembanu10@yahoo.com

3 Department of Microbiology, Saveetha University, Saveetha Nagar, Thandalam, Chennai, India. kalyanimohanram@gmail.com

*Corresponding Author E-mail: bhuvaneshwarigunasekar@gmail.com

Online published on 24 July, 2018.



Non-fermenting Gram negative bacilli (NFGNB) are the major cause of nosocomial infections worldwide. This will be life threatening, if not monitored with proper surveillance and treatment. If disinfectants are diluted, the ineffectiveness of disinfectants leads to microbial resistance.


This study was conducted to identify the disinfectant (both skin and surface disinfectant) susceptibility of NFGNB with three different concentrations.

Materials and methods

In this study, 3500 samples were cultured by agar plating technique. Out of which 240 yielded NFGNB which were identified by conventional biochemical tests. Agar cup diffusion method was done for disinfectant susceptibility testing.

Results and discussion

Among 240 NFGNB, 100% were sensitive to chlorhexidine gluconate at manufacturer's concentration. If the concentration was reduced the susceptibility percentage also was reduced. 97% and 39% of isolates were susceptible to hydrogen peroxide and povidone iodine respectively, irrespective to the concentration.


Hence, it can be stated that NFGNB has its own susceptibility to individual disinfectant at manufacturer's concentration. But none showed 100% sensitivity when the concentration was reduced than manufacturer's concentration. Thus complete surveillance and monitoring of these organisms with disinfectant suceptibility is mandatory to control the hospital acquired infections.



Non-fermenting Gram negative bacilli, disinfectant suceptibility, nosocomial infection.


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