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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 3
First page : ( 894) Last page : ( 898)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00165.8

Development and Validation of an Analytical Method for Simultaneous Estimation of Clotrimazole and its Impurity by RP-HPLC

Pandya Priyanka M., Kapupara Pankaj P., Shah Ketan V.*

School of Pharmacy, R K University, Kasturbadham, Rajkot, Gujarat, India-360020

*Corresponding Author E-mail: ketan.shah@rku.ac.in

Online published on 7 July, 2018.


A simple, efficient and reproducible method for the simultaneous determination of Clotrimazole and its Impurity (2-Chlorphenyl diphenyl methanol) has been developed using reversed phase high performance liquid chromatography. The separation was carried out using a mobile phase consisting of methanol: acetonitrile (95: 5% v/v). Column used was Zorbax C18 (100 × 4.6 mm internal diameter) 3.5 μm with flow rate of 1 ml/min. The detection wavelength used was 225 nm. The retention time of Clotrimazole and 2-Chlorophenyl diphenyl methanol were 2.81 and 2.14 respectively. Linearity of Clotrimazole and its Impurity are in range of 10–80 μg/mL and 2–12 μg/mL respectively. Analytical parameters were calculated and a statistical evaluation included.



Clotrimazole, 2-Chlorophenyl diphenyl methanol, HPLC, Simultaneous estimation, Impurity.


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