|
|
|
|
|
|
|
|
|
|
Development and Validation of an Analytical Method for Simultaneous Estimation of Clotrimazole and its Impurity by RP-HPLC Pandya Priyanka M., Kapupara Pankaj P., Shah Ketan V.* School of Pharmacy, R K University, Kasturbadham, Rajkot, Gujarat, India-360020 *Corresponding Author E-mail: ketan.shah@rku.ac.in
Online published on 7 July, 2018. Abstract A simple, efficient and reproducible method for the simultaneous determination of Clotrimazole and its Impurity (2-Chlorphenyl diphenyl methanol) has been developed using reversed phase high performance liquid chromatography. The separation was carried out using a mobile phase consisting of methanol: acetonitrile (95: 5% v/v). Column used was Zorbax C18 (100 × 4.6 mm internal diameter) 3.5 μm with flow rate of 1 ml/min. The detection wavelength used was 225 nm. The retention time of Clotrimazole and 2-Chlorophenyl diphenyl methanol were 2.81 and 2.14 respectively. Linearity of Clotrimazole and its Impurity are in range of 10–80 μg/mL and 2–12 μg/mL respectively. Analytical parameters were calculated and a statistical evaluation included. Top Keywords Clotrimazole, 2-Chlorophenyl diphenyl methanol, HPLC, Simultaneous estimation, Impurity. Top | |
|
|
|
|
║ Site map
║
Privacy Policy ║ Copyright ║ Terms & Conditions ║
 ║
|
|
|
692,622,717 visitor(s) since 30th May, 2005.
|
|
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
|
|
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.
|