The Estimation of Ceftaroline Fosamil in Lyophillized Powder for Injection by RP-HPLC. Reddy P. Govardhan1, Kumar V. Kiran1, Raju VVSS. Appala2, Ram J. Raghu3, Raju N. Appala3,* 1Department of Pharmaceutics, Unity College of Pharmacy, Raigir (V), Bhongir (M), Yadagiri(D), Telanagan State 2Deparment of Chemistry, Faculty of Pharmacy, MAHSA University, Koula Lumpur, Malaysia 3Department of Pharmaceutical Chemistry, Sultan Ul-Uloom College of Pharmacy, Mount Pleasant, 8-2-249, Road No.3, Banjara Hills, Hyderabad, Telangana-50034, India *Corresponding Author E-mail: rajnemala25@gmail.com
Online published on 12 June, 2018. Abstract A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Ceftaroline Fosamil in sterile powder for injection. An RP C-18 Hypersil BDS C18, 150x4.6 mm i.d, 5 μm partical size, with mobile phase consisting of mixture of 0.03 M ammonium acetate in water (solvent A) and methanol (solvent B) was set in the gradient mode over 30 minutes, was found to be most suitable for gradient mode was used to separate ceftaroline fosamil. The flow rate was kept at 1 ml/min and the effluents were monitored at 205 nm. The retention time was 13.12 min. The detector response was linear in the concentration of 20–240 mcg/ml. The respective linear regression equation being Y=25740x+70940. The limit of detection and limit of quantification was 0.25 and 0.75 mcg/ml respectively. The percentage assay of Ceftaroline Fosamil was 99.75%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Ceftaroline Fosamil in bulk drug and in its pharmaceutical dosage form. Top Keywords Ceftaroline Fosamil, RP-HPLC and Lyophilized powder for injection. Top |