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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 12
First page : ( 5483) Last page : ( 5489)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00999.X

Grouping and Revelation the significant Virulence genes of Escherichia coli isolated from Patients with Urinary Tract Infections

Mohammed Ghaidaa J.1,*, Abdul-Razaq Mohammed S.2

1Department of Biology, College of Science, University of Al-Qadisiyah, Al-Diwaniyah, Iraq

2Department of Microbiology, College of Medicine, University of Babylon, Babylon, Iraq

*Corresponding Author E-mail: ghaidaa.mohammed@qu.edu.iq

Online published on 18 May, 2019.

Abstract

The prevalent cause of urinary tract infections is uropathogenic Escherchia coli (UPEC), this study aimed to use polymerase chain reaction with genotyping markers; chuA, yjiA, and TspE4.C2 for grouping of Escherchia coli isolates obtained from cases of urinary tract infections of Al-Diwaniyah teaching hospital from March to August 2017, also to detect some virulence genes, fimbriae genes (fimH and papC), hemolysin(hlyA) and uropathogen specific protein(usp)gene in the particular strains. The results have been shown that all Escherchia coli isolates were backto the group B2, and the tested virulence genes were also detected in these isolates with different prevalence; fimH (100%), papC (78.57%), HlyA(100%), and usp(64.29%).From this study it has been concluded that uropathogenic Escherchia coli strains carried several virulence factors engaged the development of urinary tract infections.

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Keywords

Uropathogenic Escherichia coli, Genotyping groups, Virulence factors genes.

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