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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 12
First page : ( 5282) Last page : ( 5286)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00962.9

Biosynthesis and Characterization of Silver Nanoparticles from Aspergillus niger and its Antibacterial Activity

Dhanaraj Suresh1,4,*, Bharathiraja P.1, Dhandapani R.2, Subbaiya R.3, Kathireshan A. K.4

1Department of Biotechnology, Sengunthar Arts and Science College, Thiruchengode, Tamil Nadu, India

2School of Biological Sciences, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, India

3Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaga Mission's Research Foundation, Paiyanoor, Chennai

4Department of Microbiology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India

*Corresponding Author E-mail: sureshdhanaraj.sls@velsuniv.ac.in

Online published on 18 May, 2019.

Abstract

The fungal mediated biosynthesis of silver nanoparticles has been carried out in this study. The silver nanoparticles were synthesized from Aspergillus niger with an aqueous solution of AgNO3. The biosynthesized silver nanoparticles were characterized through UV-visible spectrophotometer, Scanning Electron microscopy, Dynamic light scattering, and Fourier Transform Infrared Spectroscopy (FTIR). The maximum absorbance was noted between 400 nm to 450 nm in visible region corresponding to the Plasmon resonance of silver nanoparticles, confirms their presence. The size and shape of the silver nanoparticles was investigated using Scanning Electron Microscopy (SEM) and spherical shaped nanoparticles were observed under SEM. The solution with Silver nanoparticles were analysed by Dynamic light scattering to analyse the particle size, and the range of single nanoparticle size was about 3nm in diameter. The stabilizing agent that keeps the silver nanoparticles as stable and dispersed was identified from FTIR spectrum, FTIR spectrum of silver nanoparticles showed three distinct peaks, 548. 38, 1636. 17 and 3347. 85cm−1. The silver nanoparticles produced from Aspergillus niger showed potential antibacterial activity against Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli, and Salmonella typhi where chloramphenicol was used as standard antibiotic.

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Keywords

Aspergillus niger, UV-vis spectrophotometer, Dynamic Light Scattering, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM).

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