Screening of Marine Actinomycetes for Fibrinolytic Enzymes Viswanathan K.*, Rebecca L. Jeyanthi Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai, Tamilnadu, India *Corresponding Author E-mail: viswanathan.bio@gmail.com
Online published on 20 December, 2018. Abstract Objective This study Aims to isolate the potential fibrinolytic enzyme from actinomycetes strains. Methods Screening of actinomycetes from marine soil samples by using starch casein agar plate method, Protease activity by skim milk agar plate method and fibrinolytic enzyme activity by Fibrin plate method. Results We have isolated 114 actinomycetes strains by starch casein agar plate method and the morphology was identified by coverslip method. Out of the 114 actinomycetes strains, 40 actinomycetes strains showed protease activity by skim milk agar plate method. Protease producing actinomycetes strains were tested for fibrinolytic activity by Fibrin plate method which was indicated by growth and clear zone of fibrinolysis. Out of 40 isolates, the fibrinolytic activity was highest in 12 actinomycetes strains. Conclusion Based on the maximum fibrinolytic activity, BN22 was found to be a potential strain for further investigation. Top Keywords Actinomycetes, Protease activity, Fibrinolytic activity. Top |