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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 7
First page : ( 2367) Last page : ( 2374)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00419.X

Biological Synthesis and Characterization of Silver Nanoparticles by Bacillus subtilis

Shaik Mahammad Rafi1,*, Dr. Pavani A.2, Dr. Rao V. Uma Maheswar3, Dr. Gupta V.R.M.1

1Department of Pharmaceutics, Pulla Reddy Institute of Pharmacy, Domadugu, Sangareddy, Hyderabad.

2Department of Pharmaceutics, Sri Venkateswara College of Pharmacy, Madhapur, Hyderabad.

3Department of Pharmacognosy, TRR College of Pharmacy, Meerpet, Hyderabad.

*Corresponding Author Email: rafeeq.prip333@gmail.com

Online published on 17 October, 2017.


Over the past few decades interest in metallic nanoparticles and their synthesis has greatly increased. This has resulted in the development of numerous ways of producing metallic nanoparticles using chemical and physical methods. However, drawbacks such as the involvement of toxic chemicals and the high-energy requirements of production make it difficult for them to be widely implemented. An alternative way of synthesizing metallic nanoparticles is by using living organisms such as bacteria, fungi and plants. The attractive possibility of green synthesis nanotechnology is to use micro organisms in the synthesis of nanoparticles. Recently, the utilization of Biological materials especially fungi and bacteria has emerged as a novel method for the development of nanoparticles. Nanoparticles are the starting points for preparing many nanostructured devices and materials. In the research we report he extracellular biosynthesis of silver nanoparticles (AgNPs) by bacteria named bacillus subtilis. In this process the toxic Ag+ ions are reduced to the non-toxic metallic AgNPs through the catalytic effect of the extracellular enzyme and metabolites of the Bacillus subtilis. Absorption UV-Visible spectroscopy is used to follow up with the reaction process. Fourier transform Infrared spectroscopy is used for quantitative analysis of the reaction products. Scanning electron microscopy study indicated that the formation of silver nanoparticles along with circular shape. Particle size analysis shows the average particle size of nanoparticles. Zeta potential indicates the surface charge of the particles and zeta potential is a powerful parameter for predicting the stability of particles. The present research is an excellent candidate for industrial scale production of silver nanoparticles.



Extracellular biosynthesis, Nanoparticles, Silver Nitrate, Bacillussubtilis, Zeta Potential.


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