Preliminary phytochemical analysis, in vitro antioxidant and anti-inflammatory activity of Turbinaria ornata (Turner) J. Agardh
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In the exploration of bioactive compounds from Turbinaria ornata a brown algae, the present study deals with preliminary phytochemicals analysis of Turbinaria ornata with different solvents along with antioxidant and anti-inflammatory activities Turbinaria ornata methanolic extract (TOME) has been investigated for its total antioxidant activity, in vitro free radical scavenging and antioxidant potential by 2, 2-diphenyl-1-pricrylhydrazyl (DPPH) scavenging assay, reducing power assay, Nitric oxide, hydrogen peroxide, superoxide scavenging assays. The anti-hemolysis and anti-inflammatory activities in RBC model of blood collected from healthy volunteers of 22–25 years age were done. The results of the present study proved that Turbinaria ornata is rich in bioactive compounds. Turbinaria ornata Methanolic extract (TOME) constitutes carbohydrates, alkaloids, saponins, phenolic compounds, flavonoids, tannins, coumarines, steroids and terpenoids. FTIR spectrum of the TOME showed the peaks at range of 3903.6, 3838.2, 3750.0, 3397.0, 2928.9, 2363.9, 1637.0, 1419.1, 1078.9 and 668.6 which are similar to the FTIR spectrum of standard gallic acid. TOME at the concentration of 100μg shows 89.11% of total antioxidant activity. The free radicals NO, H2O2 and SOD scavenging activity are increased with increase in the concentration of TOME. TOME at selected concentrations (0.5 mg/ml, 0.75 mg/ml, 1mg/ml) shows significant reduction in H2O2 induced hemolysis. Increase in TOME concentrations increased the human RBC membrane stabilization, the higher concentration level (500μg/ml) shows about 81% of anti-inflammatory activity which is significant as the standard Diclofenac. Thus Turbinaria ornata with potent bioactive compounds shows appreciable antioxidant activity, prevents H2O2 induced hemolysis and inflammation in human RBC model. Further in vivo studies, NMR assay, drug leads and molecular level investigations will be done to bring out the medicinal value of the Turbinaria ornata in treating different diseases due to the assault of free radicals and inflammation.
Anti-inflammatory activities. Turbinaria ornata methanolic extract (TOME).