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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 5
First page : ( 1471) Last page : ( 1476)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00259.1

Novel Actinomycetales bacterium-PV7 isolated from Kanyakumari Marine Sediments: A Prospective Source for Industrial and Pharmaceutical Enzyme Production

Rao K.V Bhaskara1,*, Veena S2, Pooja S2, Shriya Y2, Shivram R2

1Professor, Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore, TN-632 014, India

2Molecular and Microbiology Research Laboratory, Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore-632 014, Tamil Nadu, India

*Corresponding Author E-mail: kokatibhaskar@yahoo.co.in

Online published on 17 July, 2017.

Abstract

Research for the selection of different enzyme producing microorganisms are increased around the world. Marine actinomycetes are one of the most investigated group since they are considered as a potential source of biotechnologically interesting products. The aim of the present study was to isolate commercially useful enzymes producing marine actinobacteria from Kanyakumari marine sediments. A total of 10 actinobacterial colonies were isolated which were designated as PV-1 to PV-10. In primary screening PV1, PV5, PV7 and PV8 showed good activity for cellulase and lipase enzyme production with a zone of hydrolysis of 10 mm and above. The isolate PV7 showed maximum activity with a zone of hydrolysis of 20 mm and 19 mm for cellulase and lipase enzyme respectively. Among all isolates PV7 and PV10 showed higher protease enzyme production with a zone of hydrolysis 19 mm and 14 mm respectively. In secondary screening PV7 showed maximum activity of 18.65 U/ml for cellulase followed by 15.32 U/ml of protease and 11.34 U/ml of lipase activities. The potential isolate PV7 was identified as Actinomycetales bacterium-PV7 using molecular methods by 16S rRNA sequencing. This study concludes that marine actinobacterial can be considered as source for the production of industrial and pharmaceutical enzymes.

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Keywords

Marine actinobacteria, Lipase, Protease, Cellulase, Actinomycetales bacterium.

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