Effect of Solvents on Spectrophotometric Determination of Cefpodoxime Proxetil in pure and tablet dosage form through Ion-Pair Complex Formation using Bromophenol blue Ramadan Abdul Aziz*, Mandil Hasna, Shamseh Rasha Department of Chemistry, Faculty of Sciences, University of Aleppo, Syria *Corresponding Author E-mail: dramadan1946@gmail.com
Online published on 17 July, 2017. Abstract A simple, direct and accurate spectrophotometric method has been developed for the determination of cefpodoxime proxetil (CEFP) in pure and tablet dosage form. The method involves the formation of yellow ion-pair complexes between the cited drug and bromophenol blue (BPB) using some solvents. Dichloroethane and chloroform were the best. The absorbance is measured at λmax 412 and 422 nm in dichloroethane and chloroform, respectively. Variables were studied in order to optimize the reaction conditions. Molar absorptivity (ε) for [CEFP]: [BPB] and [CEFP]: [BPB]2 complexes were selected. Beer's law was obeyed in the concentration range of 0.2788–44.608 μg.mL−1 and 0.5576–44.608 μg.mL−1 in presence of 1 x 10−3 mol. L−1 of BPB with good correlation coefficient (R2= 0.9996 and R2= 0.9998) in dichloroethane and chloroform, respectively. The relative standard deviation did not exceed 4.3%. The proposed method was validated for specificity, linearity, precision and accuracy, repeatability, sensitivity (LOD and LOQ) and robustness. The developed method is applicable for the determination of CEFP in pure and different dosage forms with average recovery 98.8% to 101.1%, and 98.6% to 101.2% in dichloroethane and chloroform, respectively, and the results are in good agreement with those obtained by the RP-HPLC reference method. Top Keywords Direct spectrophotometric method; Cefpodoxime proxetil; Bromophenol Blue; Ion-pair complex. Top |