Larvicidal Activity of Ethanol Leaf Extract of Pinus merkusii on Aedes aegypti larvae Sudjarwo Sri Agus1,*, Ngadino2, Koerniasari2, Setiawan2, Sudjarwo Gifta Wardani3 1Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia 2Study Program of Environmental Health, Polytechnic of Health, Surabaya, Indonesia 3Faculty of Pharmacy, Hang Tuah University, Surabaya, Indonesia *Corresponding Author E-mail: ags158@yahoo.com
Online published on 17 July, 2017. Abstract The mosquito species, Aedes aegypti L. is a vector of major diseases such as dengue haemorrhagic fever, chikungunya and yellow fever. Therefore, it is necessary to find new bioinsectiside which is expected to have larvicidal effects. This study investigates ethanol leaf extracts of Pinus merkusii for efficacy against Aedes aegypti larvae. This experiment using a completely randomized design with 8 treatment groups. Each group contained five times repetition using 20 third instar larvae of Aedes aegypti with concentrations of Pinus merkusii leaf extract are 0, 10, 20, 40. 80, 160, 320, 640 ppm. Larval mortality was observed for 24 h, LC50 and LC90 were analyzed using Probit analysis. Ethanol leaf extract of Pinus merkusii showed highest larvae mortality against the larvae of Aedes aegypti with LC50 = 145.7 ppm; LC90 = 379.4 ppm after 12 h, and LC50 = 86.3 ppm; LC90 = 173.6 ppm after 24 h. These findings suggest that ethanol leaf extracts from Pinus merkusii have larvicidal activity that can be exploited in development of new bioinsecticides. Top Keywords Extract Pinus merkusii leaf, Larvicidal, Aedes aegypti. Top |