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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 2
First page : ( 557) Last page : ( 561)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00111.1

Simultaneous UPLC Estimation of Fexofenadine HCl and Montelukast Sodium Tablets

Mustafa Mohamed, Amuthalakshmi S.*, Nalini C.N.

Department of pharmaceutical analysis, C.L. Baid Metha College of Pharmacy, Thorapakkam, Chennai-600097

*Corresponding Author E-mail: amuthaaris@gmail.com

Online published on 29 April, 2017.

Abstract

A new simple, rapid and sensitive Gradient Ultra Performance Liquid Chromatography (UPLC) method has been developed for the determination of Fexofenadine HCl and Montelukast Sodium. The method employs Thermo Scientific UPLC system on Waters (symmetry) (c18 1.8 micron 4.6 x 50mm) column. Best chromatographic separation was achieved by using acetonitrile: 20 mM potassium dihydrogen phosphate 80: 30 (v/v) adjusted to pH 5.5 using orthophosphoric acid as mobile phase at a flow rate of 1 ml/min and detection at 230 nm. Separation was completed within 10 min. The retention time of Fexofenadine HCl and Montelukast Sodium was found to be 1.022 and 3.281. The proposed method was found to have linearity in concentration range of 80–120 μg/ml and 96–144 μg/m. The developed method has been statistically validated and was found to be simple, precise, reproducible and accurate. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage form.

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Keywords

Fexofenadine HCl, Montelukast Sodium, UPLC estimation, Analytical method validation.

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