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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 12
First page : ( 4178) Last page : ( 4182)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00762.4

Analyzing the correlation of TLR-4 Asp299Gly and Thr399Ile polymorphism in women with Bacterial Vaginosis in Hillah city.

Bunyan Ilham A.1, Umran Bushra J.2, Salman Zahraa Kais3,*

1Department of Microbiology, College of Medicine, University of Babylon, Hillah, Iraq

2Department of Gynecology and Obstetrics, College of Medicine, University of Babylon, Hillah, Iraq

3Department of Microbiology, College of medicine, University of Babylon, Hillah, Iraq

*Corresponding Author E-mail: Ilhamalsaedi2008@gmail.com

Online published on 26 March, 2018.

Abstract

Women in fecundity age have different vaginal infections, the commonly is bacterial vaginosis. Different microorganisms causes bacterial vaginosis, One of them were anaerobes that considered as vaginal flora and there presents in large number due to decrease in Lactobacilli growth lead to causing vaginal infections, where toll-like receptor-4 (TLR-4) can be stimulating by induces of immune response with these microbes. The association of TLR4polymorphism with the incidence of bacterial vaginosis among women was investigated in this study. High vaginal swabs and blood samples were obtained from 150 married women plagued from bacterial vaginosis and 150 unailing ladies as control. Human DNA was extracted, and TLR4 gene was amplified using specific primers. Restriction fragment length polymorphism technique was used for genotyping. Two single nucleotide polymorphisms (SNPs) (Asp299Gly and Thr399Ile) were investigated their relation with bacterial vaginosis. There is insignificant association between Thr399Ile and bacterial vaginosis (OR=0.765, 95%CI= 0.319–1.834, p= 0.001).

The SNPs of Thr399Ile canbe indicated to the susceptibility to get bacterial vaginosis.

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Keywords

Bacterial Vaginosis, Toll-like receptor 4, polymorphism, restriction enzyme, Asp299Gly, Thr399Ile.

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