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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 10
First page : ( 3391) Last page : ( 3397)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00603.5

Determination of Antioxidant Activity of various extracts of Pajanelia longifolia (Willd.) K. Schum, isolation and characterization of Flavonoid from Ethanol extract by Column Chromatography

Gini E J1,*, Sivakkumar T2, Kuppuswami S3

1Chemists College of Pharmaceutical Sciences and Research, Varikoli, Kerala, India-682308

2Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India-608002

3Department of Pharmaceutics, Nirmala College of Pharmacy, Muvattupuzha, Kerala, India-686661

*Corresponding Author E-mail:

Online published on 26 March, 2018.

Abstract

The present study involves the determination of antioxidant activity of aqueous and ethanolic extracts of the plant Pajanelia longifolia (Willd.) K. Schum, isolation and characterization of flavonoid from the ethanolic extract by column chromatography. DPPH assay, ABTS assay, hydroxyl radical scavenging activity, super oxide free radical scavenging activity, nitric oxide radical scavenging activity and total antioxidant activity methods were used to study the antioxidant potential of the whole plant. Among the two extracts, the alcoholic extract shows better antioxidant activity. The alcoholic extract was subjected to column chromatography by gradient elution technique and the fraction 98–102 was found to be 3 ’, 4 ’, 5, 7-tetrahydroxy-3-o-rhamnoglucosyl flavonone.

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Keywords

Pajanelia longifolia (Willd.) K. Schum, column chromatography, DPPH assay, ABTS assay, hydroxyl radical scavenging activity, super oxide free radical scavenging activity, nitric oxide radical scavenging activity, total antioxidant activity, 3 ’, 4 ’, 5, 7-tetrahydroxy-3-o-rhamnoglucosyl flavonone.

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