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Research Journal of Pharmacognosy and Phytochemistry
Year : 2018, Volume : 10, Issue : 1
First page : ( 15) Last page : ( 22)
Print ISSN : 0975-2331. Online ISSN : 0975-4385.
Article DOI : 10.5958/0975-4385.2018.00003.1

Phytochemical analysis and biomass estimation of Chaetomium cupreum extracts through submerged fermentation (SmF)

Wani Nazir Ahmad, Tirumale Sharmila*

Department of Microbiology and Biotechnology, Jnanabharathi Campus, Bangalore University, Bengaluru-560056, Karnataka, India

*Corresponding Author E-mail: sharmilabub@gmail.com

Online published on 24 May, 2018.

Abstract

The aim of the study was extraction of extracellular biopigments from C. cupreum and its effect on pigment production, biomass yield and phytochemical analysis. The submerged fermentation method was used for high pigment production. The dry yield obtained for chloroform extract was 26.85 mg, ethyl acetate extract 120.06 mg, n-butanol extract 69.85 mg and methanol extract 176.73 mg was obtained per gram of biomass per litre. The phytochemical analysis showed C. cupreum extracts contains different classes of natural products such as flavonoids, saponins, carbohydrates, glycosides, tannins, phytosterol, phenolic, terpenoid and coumarins compounds whereas alkaloids were found to be absent. The obtained results suggest that C. cupreum extracts contain different classes of pigments useful for the various industrial applications after their purification and characterization.

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Keywords

Chaetomium cupreum, submerged fermentation, extraction method, pigment yield, azaphilones, phytochemical analysis.

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