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Research Journal of Pharmaceutical Dosage Forms and Technology
Year : 2020, Volume : 12, Issue : 1
First page : ( 27) Last page : ( 32)
Print ISSN : 0975-234X. Online ISSN : 0975-4377.
Article DOI : 10.5958/0975-4377.2020.00005.1

Review on Chromatography Principal Types and it's Application

Patil Ashwini. R.*, Ghagare Prajkta. M., Deshmane Bhavna. J., Kondawar Manish. S.

Department of Pharmaceutical Chemistry, Appasaheb Birnale College of Pharmacy, Sangli

*Corresponding Author E-mail: ashwinipatil9621@gmail.com

Online published on 10 August, 2020.

Abstract

Chromatography is a method of separation. Chromatography is a major biophysical technology to isolate, identify and purify the components of a qualitative and quantitative blend study. The compound is dissolved during the oil moving process. That carries it through a system that holds another material named stationary phase. a Separation depends on the relative bility of the component Adsorbing and/or partitioning entre phase mobile and phase stationary. And the procedure is categorized in different types depending on the theory of separation, process geometry, chromatography mode. For stationary bed-based separation technique including column, thin layer and chromatography on paper. Chromatography of affinity often uses a metal affinity of the biomolecules. A major qualitative and quantitative technique is the high-performance liquid chromatography (HPLC). It is used for pharmaceutical and biologic sample estimation. Quality control of product components is the most flexible, easiest, efficient and quickest chromatography technique.

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Keywords

Gas chromatography, liquid chromatography, thin layer chromatography High-Performance Liquid Chromatography.

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