Review on Chromatography Principal Types and it's Application Patil Ashwini. R.*, Ghagare Prajkta. M., Deshmane Bhavna. J., Kondawar Manish. S. Department of Pharmaceutical Chemistry, Appasaheb Birnale College of Pharmacy, Sangli *Corresponding Author E-mail: ashwinipatil9621@gmail.com
Online published on 10 August, 2020. Abstract Chromatography is a method of separation. Chromatography is a major biophysical technology to isolate, identify and purify the components of a qualitative and quantitative blend study. The compound is dissolved during the oil moving process. That carries it through a system that holds another material named stationary phase. a Separation depends on the relative bility of the component Adsorbing and/or partitioning entre phase mobile and phase stationary. And the procedure is categorized in different types depending on the theory of separation, process geometry, chromatography mode. For stationary bed-based separation technique including column, thin layer and chromatography on paper. Chromatography of affinity often uses a metal affinity of the biomolecules. A major qualitative and quantitative technique is the high-performance liquid chromatography (HPLC). It is used for pharmaceutical and biologic sample estimation. Quality control of product components is the most flexible, easiest, efficient and quickest chromatography technique. Top Keywords Gas chromatography, liquid chromatography, thin layer chromatography High-Performance Liquid Chromatography. Top |