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Research Journal of Engineering and Technology
Year : 2016, Volume : 7, Issue : 4
First page : ( 156) Last page : ( 166)
Print ISSN : 0976-2973. Online ISSN : 2321-581X.
Article DOI : 10.5958/2321-581X.2016.00028.3

Effect of sub lethal exposure of LC50 of butachlor on the population dynamics of earthworm Eutyphoeus waltoni

Singh Vandana, Singh Keshav*

Vermibiotechnology Laboratory, Department of Zoology, D.D.U. Gorakhpur University, Gorakhpur, 273 009, Uttar Pradesh, India

*Corresponding Author Email: keshav26singh@rediffmail.com

Online published on 17 July, 2017.


The sub-lethal exposure (20% and 60%) of LC50 of herbicide butachlor of different feed materials of buffalo dung with agro-wastes of different exposure periods was observed in the laboratory. There was time and dose dependent Sub-lethal (20% and 60%) of LC50 of different exposure periods were observed after treatment of different combination of buffalo dung with agro-wastes. The prolong duration in clitellum development (days), significant reduction in cocoon production/worm and hatchlings/cocoon as well as survival of hatchlings were observed. The sub lethal effect of herbicide butachlor on the earthworm Eutyphoeus waltoni was both time and dose dependent. The prolong duration of clitellum development was observed in 60% LC50 of all exposure periods in buffalo dung. Maximum per cenatage of cocoon production, hatchling/cocoon and survival of hatchling was observed in 20% LC50 of all exposure periods in buffalo dung, wheat straw and gram bran combination. There was no mortality observed in control. There was no significant (P< 0.05, t-test) difference between control and 20% of LC50 of BD+Ws+Gb of all exposure periods shows that the vermicompost of feed material of this combination was more potential for use in agricultural fields because it enhance the tolerance power of earthworm Eutyphoeus waltoni against herbicide butachlor.



Herbicide, Butachlor, buffalo dung, Agro-wastes, Sub lethal exposure, Eutyphoeus waltoni, Reproduction, Development.


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