Molecular identification and primer design for spotted wing drosophila (Drosophila suzukii) Naserzadeh Yousef1,2,*, Mahmoudi Niloufar1,2, Pakina Elena1, Zargar Meisam1 1Department of Agrobiotechnology, Institute of Agriculture, RUDN University, Moscow, 117198, Russia 2Researcher, All-Russian Plant Quarantine Centre (VNIIKR), Moscow, Russia * (e-mail: unaserzadeh@gmail.com)
Online published on 20 July, 2020. Abstract Spotted wing drosophila (Drosophila suzukii) belonging to the family Drosophilidae is a harmful insect pest in Russian Federation. At times, it may be specific to classify using adults and larva or nymph samples. The method of molecular diagnosis for D. suzukii has been developed from another non-quarantine species Drosophila sp. using the mitochondrial DNA cytochrome oxidase I region of 709-bp. DNA samples with LCO1490 and HCO2198 primers amplified by Drosophila spp. collections in laboratory samples from Mexico, Egypt and Turkey were compared to other GenBank Drosophila taxa sequences. Based on DNA sequence polymorphisms, the findings of the polymerase chain reaction (cPCR) have shown that these primers (12. dsuz.F/R) classify the exact region of the gene as well as the specific Drosophila suzukii primer. This DNA collection is appropriate for regular use by diagnostic and research organizations as safety measure in quarantine and further reduce the spread of this pest. Top Keywords Drosophila suzukii, molecular identification, PCR, plant quarantine, primer design. Top |