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Research on Crops
Year : 2015, Volume : 16, Issue : 1
First page : ( 195) Last page : ( 199)
Print ISSN : 0972-3226. Online ISSN : 2348-7542.
Article DOI : 10.5958/2348-7542.2015.00028.5

Determination of saikosaponins in three Bupleurum plants by HPLC analysis

Kim Hyeusoo, Kim Sea Hyun, Huh Chang-Ki1, Seo Kyoung-Sun2, Park Tae-Young2, Yun Kyeong Won3,*

Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon-441–847, Republic of Korea

1Imsil Research Institute of Cheese Science, Imsil 566–881, Republic of Korea

2Jangheung County Mushroom Research Institute, Jangheung 529–851, Republic of Korea

3Department of Oriental Medicine Resources, Sunchon National University, Suncheon 540–950, Republic of Korea

*E-mail: ykw@sunchon.ac.kr

Online published on 30 March, 2015.

Abstract

In this study, saikosaponins were determined from roots of three Bupleurum plants collected in June 2009. Bupleuri Radix (Bupleurum spp. root) is one of the most important crude drugs in Korea, China and Japan. High performance liquid chromatography (HPLC) was used for the determination of saikosaponin ‘a’, ‘b’ and ‘c’ in three Bupleurum plants. The highest total saikosaponin content was found in the B. falcatum ‘Mishima’ (1281.94 mg%). Total saikosaponin contents of B. falcatum and B. latissimum were 669.67 and 489.95 mg%, respectively. B. falcatum ‘Mishima’ and B. falcatum contained more saikosaponin ‘a’ than other saikosaponins. The contents of saikosaponin ‘a’ of B. falcatum ‘Mishima’ and B. falcatum were 745.74 and 484.60 mg%, respectively. In contrast, B. latissimum, an endemic species of Korea had higher saikosaponin ‘c’ (351.90 mg%) content than the other two saikosaponins and the content was higher than B. falcatum (181.71 mg%). B. latissimum, a Korean endemic species, is a good source for extraction/production of saikosaponin ‘c’.

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Keywords

Bupleurum falcatumBupleurum falcatum ‘Mishima’, Bupleurum latissimum, Korean endemic species, saikosaponin.

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