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ORYZA- An International Journal on Rice
Year : 2016, Volume : 53, Issue : 2
First page : ( 126) Last page : ( 135)
Print ISSN : 0474-7615. Online ISSN : 2249-5266.

Isolation and characterization of the promoter of RGG1 using Agrobacterium-mediated transient assay

Swain Durga Madhab1,4, Sahoo Ranjan Kumar1, Tripathy Baishnab Charan2,4, Tuteja Narendra1,3,*

1International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India

2Jawaharlal Nehru University, New Delhi-110067, India

3Amity University, Noida-201313, India

4Ravenshaw university, Cuttack, Odisha-753003

*Corresponding author e-mail: narendra@icgeb.res.in; ntuteja@amity.edu

Gene Bank Accession Number of RGG1: GU111573.1

Online published on 8 February, 2017.


The heterotrimeric G protein complex, comprising of G α, G β, and G γ subunits, is an evolutionarily conservedsignaling molecular machine which transmits signals from transmembrane receptors to downstream target proteins. Now-a-days their functions in plant stress-signalling have been reported. Here we have isolated the promoter of rice G-protein γ subunit 1(RGG1) from rice genomic DNA. In silico analysis revealed that promoter of RGG1 contained a TATA, a CAAT motif and also harbors some important stress and hormone associated cis regulatory elements, including E-box, GAAAAA, GATA-box, CACATG, CCGTCC and CCACC. Functional analyses were performed by Agrobacterium-mediated transient assay in tobacco leaves. Very high level of GUS activity was observed in agro infiltrated tobacco leaves by the construct carrying the RGG1 promoter:: GUS, subjected to abiotic stress and exogenous hormonal treatments. Stress-inducible nature of RGG1 promoter opens possibility for the study of the gene regulation under stress condition. Therefore, the result may be useful in the field of agriculture and biotechnology



Cis acting elements, RGG1 promoter, Place database, Agroinfiltration, GUS activity.


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