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Medicinal Plants - International Journal of Phytomedicines and Related Industries
Year : 2013, Volume : 5, Issue : 4
First page : ( 202) Last page : ( 205)
Print ISSN : 0975-4261. Online ISSN : 0975-6892.
Article DOI : 10.5958/j.0975-6892.5.4.032

Evaluation of phytochemicals and in vitro antioxidant studies of Toddalia asiatica leaf

Ceballos Rachael1, Sankoli Seethalakshmi Madhukar3, Ramnath Shubharani2, Venkataramegowda Sivaram4,*, Murthy Ganti S.5, Ceballos Ruben Michael6

1DVM/PHD, The University of Minnesota (Minneapolis/St.Paul, USA

2Department of Botany, Bangalore University, Bangalore-560056, India

3V Sivaram Research Foundation, # 132, 2nd Main Road, R R Layout, Bangalore, India

4University of Minnesota, Morris, MN, USA

5Department of Biological & Ecological Engineering, Oregon State University, Corvallis, OR, USA

6Native American Research Laboratory, Division of Science and Mathematics, University of Minnesota, Morris, MN, USA

*Corresponding author: Sivaram Venkataramegowda e-mail: sivaram900@gmail.com

Abstract

The primary phytochemical and in vitro antioxidant studies were performed on methanol, chloroform, petroleum ether and aqueous leaf extracts of Toddalia asiatica. The antioxidant activity of methanolic leaf extract was determined by DPPH free radical scavenging assay. Ascorbic acid was used as standard. Evaluation showed high phenol content and methanolic extract exhibited significant antioxidant activity. The DPPH radical scavenging activity of the extract was increased with the increasing concentration. The highest radical scavenging effect was observed with IC50 = 36μg/ml. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by Toddalia asiatica leaf extract. The results concluded that the plant has potential source of antioxidants of natural origin, augmenting its therapeutic value.

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Keywords

Antioxidant, Toddalia asiatica, flavonoids, phenols.

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