Evaluation of phytochemicals and in vitro antioxidant studies of Toddalia asiatica leaf Ceballos Rachael1, Sankoli Seethalakshmi Madhukar3, Ramnath Shubharani2, Venkataramegowda Sivaram4,*, Murthy Ganti S.5, Ceballos Ruben Michael6 1DVM/PHD, The University of Minnesota (Minneapolis/St.Paul, USA 2Department of Botany, Bangalore University, Bangalore-560056, India 3V Sivaram Research Foundation, # 132, 2nd Main Road, R R Layout, Bangalore, India 4University of Minnesota, Morris, MN, USA 5Department of Biological & Ecological Engineering, Oregon State University, Corvallis, OR, USA 6Native American Research Laboratory, Division of Science and Mathematics, University of Minnesota, Morris, MN, USA *Corresponding author: Sivaram Venkataramegowda e-mail: sivaram900@gmail.com
Abstract The primary phytochemical and in vitro antioxidant studies were performed on methanol, chloroform, petroleum ether and aqueous leaf extracts of Toddalia asiatica. The antioxidant activity of methanolic leaf extract was determined by DPPH free radical scavenging assay. Ascorbic acid was used as standard. Evaluation showed high phenol content and methanolic extract exhibited significant antioxidant activity. The DPPH radical scavenging activity of the extract was increased with the increasing concentration. The highest radical scavenging effect was observed with IC50 = 36μg/ml. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by Toddalia asiatica leaf extract. The results concluded that the plant has potential source of antioxidants of natural origin, augmenting its therapeutic value. Top Keywords Antioxidant, Toddalia asiatica, flavonoids, phenols. Top |