Micropropagation, antimicrobial activity and phytochemical analysis of Clerodendron phlomidis Linn. Raaman Nanjian1, Divakar Samidurai, Jeyam Palanivel, Hariprasath Lakshmanan, Baskar Mohan, Mathiyazhagan Kasinathan Herbal Sciences Laboratory, Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-600025, India 1Corresponding author: N. Raaman raaman55@gmail.com
Abstract Clerodendron phlomidis is a traditional medicinal plant used in folk medicines. In the present study, the successive in-vitro micropropagation of C. phlomidis was optimized in Murashige and Skoog (MS) medium with addition of different plant growth regulators (PGR) individually and in combination. Interestingly, NAA at 3 mg/L showed effective callus production (97%) of leaf explants whereas combination of IAA, IBA and NAA at the level of 0.5, 0.1 and 0.1 mg/L respectively, resulted in a significant callus production (51%) from the nodal and internodal explants. The organogenic callus of C. phlomidis produced multiple shoots (87%) in the MS medium amended with BAP, KIN, and NAA 2.5, 1.5 and 0.1 mg/L respectively. In addition, the antimicrobial activity of different organic solvent extracts of C. phlomidis was tested against human as well as plant pathogens. The hexane extract at 150 µg/mL revealed effective inhibitory activity to all the test pathogens, however, chloroform and methanol extracts showed moderate antimicrobial activity. Furthermore, the phytochemical ingredients of all the extracts were screened qualitatively by thin layer chromatography (TLC). Top Keywords Clerodendron phlomidis, Micropropagation, Plant growth regulators, Antimicrobial activity, Phytochemicals. Top |