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Medicinal Plants - International Journal of Phytomedicines and Related Industries
Year : 2009, Volume : 1, Issue : 2
First page : ( 97) Last page : ( 101)
Print ISSN : 0975-6892.
Article DOI : 10.5958/j.0975-4261.1.2.012

Quantitative assessment of xanthone derivatives in Swertia chirata (Wall.) by RP-HPLC with UV detection

Negi Jagmohan S., Singh Pramod, Pant Geeta Joshi nee*, Rawat M.S.M.

Department of Chemistry, HNB Garhwal University, Srinagar Garhwal - 246 174, India.

*Corresponding author: Geeta Joshi nee Pant, E-mail: geeta_joshi4f54@rediffmail.com

Abstract

The chromatographic profile of xanthones found in Swertia chirata was studied by HPLC using a C18 reversed phase column and aqueous methanol mobile phase using UV absorption at 260 nm. RP-HPLC method was developed and validated for the determination of two xanthone derivatives, 2, 8-dihydroxy-1, 6-dimethoxyxanthone (SR1) and 1, 2, 6, 8-tetrahydroxyxanthone (SR2) in leaves and rhizomes of S. chirata. The HPLC fingerprints of four batches of herbal samples from different regions of Uttarakhand Himalaya showed similar chromatographic patterns. The retention time of markers peak in the HPLC fingerprint were determined as an important parameter for identification. In this herb, SR1 and SR2 were quantitatively determined using a validated reversed phase HPLC method. The bioactives of S. chirata were quantified and their variation was studied in cultivated and wild species with season and altitude to infer about prime of potency and proper harvesting period. The limit of detection obtained for the analytes were in the range of 0.86–2.24 ppm and the maximum concentration of xanthones was found in cultivated plants.

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Keywords

HPLC, Swertia chirata, xanthone derivatives, quantitative determination.

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