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Phytopathogenic Mollicutes
Year : 2015, Volume : 5, Issue : 1-Suppl.
First page : ( S23) Last page : ( S24)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/2249-4677.2015.00009.2

Saving livestock fodder in East Africa: development of a rapid penside diagnostic assay for detection of napier grass stunt phytoplasma

Wambua Lillian1,*, Schneider Bernd2, Fischer-Jores Anne1

1International Centre for Insect Physiology and Ecology, P.O Box 30772, 00100, Nairobi, Kenya

2Julius Kuhn Institut, Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101 69221, Dossenheim, Germany

*Corresponding author e-mail: Lillian Wambua (lwambua@icipe.org)

Online published on 2 January, 2015.

Abstract

Napier grass stunt is a disease associated with phytoplasmas belonging to the 16SrXI subgroup. The disease affects Pennisetum purpureum, a perennial grass species with significant importance in dairy livestock nutrition and ecological management of pests and parasitic weeds. The present study explores the development, testing and validation of field-friendly diagnostic tools for napier grass stunt phytoplasma detection in East Africa, based on an isothermal assay targeting the gene encoding the immunodominant protein of napier grass stunt phytoplasma.

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Keywords

Napier grass stunt, phytoplasma, diagnostic assay, imp gene.

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