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Phytopathogenic Mollicutes
Year : 2023, Volume : 13, Issue : 1
First page : ( 79) Last page : ( 80)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/2249-4677.2023.00040.3

Multilocus RFLP characterization of ‘Candidatus Phytoplasma pyri’ strains in pear from Chile and Italy

Zamorano Alan1, Gamboa Camila1, Fuentes Javiera1, Cabrera Sebastián1, Herrera Camila1, Bianco Pietro2, Bertaccini Assunta2, Fiore Nicola1,*

1Universidad de Chile, Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, La Pintana, Santiago, Chile

2Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, Italy

*Corresponding author e-mail: Nicola Fiore (nfiore@uchile.cl)

Online published on 23 May, 2023.

Abstract

Candidatus Phytoplasma pyri’ associated with pear decline in Italy and Chile severely affects orchards. To its molecular characterization, results of multilocus RFLP analyses on seven phytoplasma genes (16Sr, aceF, secY, leu, secA, tuf, and lsu 36p) resulted a promising tool. To complete this work and better understand the discrimination ability of the used genes the amplified DNAs obtained from Chilean and Italian strains will further be cloned and sequenced.

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Keywords

Pear decline, Nested-PCR, Phytoplasma genes, 16SrX-C.

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