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Year : 2013, Volume : 2, Issue : 1
First page : ( 1) Last page : ( 6)
Print ISSN : 2319-118X. Online ISSN : 2319-1198. Published online : 2013 April 1.
Article DOI : 10.5958/j.2319-1198.2.1.001

Estimation of Genetic Diversity Based on Seed Storage Proteins among Different Varieties of Trigonella foenum-graecum

Hora Amandeep1,**, Chittora Manish2, Malik CP3,*

1 Ph.D Scholar, School of Life Sciences, Institute of Genetics and Hospital for Genetic Diseases, Jaipur, Rajasthan, India

2 Faculty, School of Life Sciences, Institute of Genetics and Hospital for Genetic Diseases, Jaipur, Rajasthan, India

3 Advisor (Academics), School of Life Sciences, Institute of Genetics and Hospital for Genetic Diseases, Jaipur, Rajasthan, India

* Email id: cpm_malik@yahoo.com

**aman.hora87@gmail.com

Abstract

A quantitative categorization of seed storage proteins profiles of 8 varieties of Trigonella foenum graecum was performed by sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE). This technique was used to explore the level of genetic discrepancy in Trigonella. Total soluble proteins were resolved on 10% resolving gel. A total of 25 polypeptide bands were obtained among which 21 bands were present in all the varieties but other 4 bands of molecular weights (87.096, 35.121, 28.540 and 19.412 KDa) exhibited variation. Dendrogram based on electrophoretic data clustered the varieties mainly into two groups. In conclusion, electrophoretic bands (SDS-PAGE) of seed storage proteins can provide a potent tool to estimate genetic variation and relation among germplasm. The specific bands of seed storage protein profiles may be used as markers for identification of the varieties.

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Keywords

Genetic diversity, SDS-PAGE, Seed Protein, Trigonella foenum graecum.

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