Estimation of Genetic Diversity Based on Seed Storage Proteins among Different Varieties of Trigonella foenum-graecum Hora Amandeep1,**, Chittora Manish2, Malik CP3,* 1 Ph.D Scholar, School of Life Sciences, Institute of Genetics and Hospital for Genetic Diseases, Jaipur, Rajasthan, India 2 Faculty, School of Life Sciences, Institute of Genetics and Hospital for Genetic Diseases, Jaipur, Rajasthan, India 3 Advisor (Academics), School of Life Sciences, Institute of Genetics and Hospital for Genetic Diseases, Jaipur, Rajasthan, India * Email id: cpm_malik@yahoo.com
**aman.hora87@gmail.com
Abstract A quantitative categorization of seed storage proteins profiles of 8 varieties of Trigonella foenum graecum was performed by sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE). This technique was used to explore the level of genetic discrepancy in Trigonella. Total soluble proteins were resolved on 10% resolving gel. A total of 25 polypeptide bands were obtained among which 21 bands were present in all the varieties but other 4 bands of molecular weights (87.096, 35.121, 28.540 and 19.412 KDa) exhibited variation. Dendrogram based on electrophoretic data clustered the varieties mainly into two groups. In conclusion, electrophoretic bands (SDS-PAGE) of seed storage proteins can provide a potent tool to estimate genetic variation and relation among germplasm. The specific bands of seed storage protein profiles may be used as markers for identification of the varieties. Top Keywords Genetic diversity, SDS-PAGE, Seed Protein, Trigonella foenum graecum. Top |