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Legume Research - An International Journal
Year : 2021, Volume : 44, Issue : 12
First page : ( 1493) Last page : ( 1496)
Print ISSN : 0250-5371. Online ISSN : 0976-0571.
Article DOI : 10.18805/LR-4248

Screening lentil (Lens culinaris Medik) Genotypes for Resistance against Pre-Flowering Blight and Identification of Pathogen by ITS Sequencing

Roy Anirban, Das Camellia, Sarkar Moutushi, Mondal Sourav, Ganguly Shamba, Murmu Sumit Kumar, Nath Birendra, Panja, Nath Rajib, Tripath Kuldeep1, Bhattacharyya Prabir Kumar, Bhattacharyya Somnath*

Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741 252, West Bengal, India.

1National Bureau of Plant Genetic Resources, New Delhi-110 012, India.

*corresponding author: Somnath Bhattacharyya, Department of Genetics and Plant Breeding, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia-741252, West Bengal, India. Email: smnthbhat@gmail.com

Online published on 27 January, 2022.

Abstract

Major constraints in increasing lentil growing area in eastern India is mortality due to several diseases throughout the growth stages. Breeding lentil for disease tolerance by genotypic resistance is most promising and easier method. Therefore, a collection of 500 genotypes have been screened in this study for pre-flowering blight-like disease which is predominant in experimental field for last ten years. Fungal DNA isolation from ten randomly selected diseased plants followed by ITS sequencing using universal primer pair revealed cent percent similarity in NCBI database with single organism Alternaria alternata. Three popular varieties viz. WBL-77, Ranjan and Asha were kept as check for disease reaction comparison. 466 genotypes survived till flowering and scoring was made using 1-9 scale. Only three lines, EC866132,IC267 67, IC201778 established as the tolerant lines against Alternaria blight attack at the pre-flowering stage.

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Keywords

Alternaria, Blight, ITS, Lentil.

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