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Year : 2019, Volume : 19, Issue : 1
First page : ( 51) Last page : ( 54)
Print ISSN : 0972-5687. Online ISSN : 0974-083X. Published online : 2019 June 1.
Article DOI : 10.5958/0974-083X.2019.00011.6

Amel Gene Profiling from Toothbrush for Sex Determination Among Tanzanians in Surabaya, East Java.

Nzilibili Simon Martin Manyanzaa,b,*, Yudianto Ahmada,c,d

a. M Sc. Forensic Science Student Forensic Science Program, Post Graduate School, Airlangga University, Surabaya – Indonesia

b. M Sc. Forensic Science Student Ministry of Health, Community Development, Gender Elderly and Children, Dodoma – Tanzania

c. Lecturer Department of Forensic and Medico-legal, Faculty of Medicine, Airlangga University, Surabaya – Indonesia

d. Lecturer Human Genetic Laboratory, Institute of Tropical Disease, Airlangga University, Surabaya – Indonesia

* Corresponding Author Simon Martin Manyanza Nzilibili Forensic Science Program, Postgraduate School, Airlangga University, 4–6 Airlangga Rd., 60286 Surabaya, Indonesia Contact: +62 81334326515 E-mail: nzilibili@gmail.com

Received:  18  September,  2018; Revised:  18  November,  2018; Accepted:  18  November,  2018; :  1  June,  2019.

Abstract

Introduction: Forensic identification depends significantly on evidence detected at the crime scene. Toothbrush continues to prove its usability to aid the identification for sex determination. Its contributory power continues to be confirmed to the different population at the varied time.

Materials and Methods: The present study reports the use of toothbrush in determining sex through Amel gene method by DNA band contrast at 106bp and 112bp among Tanzanians on ten volunteers.

Results: Electrophoresis band contrast confirmed six samples to be of male origin and three sample for the female. One sample presented undetectable DNA band contrast by showing the blank image on F1 sample profiled.

Conclusion: Toothbrush as source of biological samples and Amelogenin gene through DNAband contrast analysis is helpful in sex determination among Tanzanians. This finding not only evidently proposes but also contributes to bench marking applicability of Amel gene analysis as a successful and reliable method of sex determination to localized population (Tanzanians) for strengthened evidential data for forensic service.

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Keywords

Amel Gene, Toothbrush Evidence, Sex Determination.

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