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Journal of Meat Science
Year : 2018, Volume : 13, Issue : 2
First page : ( 30) Last page : ( 33)
Print ISSN : 0975-5209. Online ISSN : 2581-6616.
Article DOI : 10.5958/2581-6616.2018.00017.8

Estimation of Conjugated Linoleic Acid (CLA) Content in Ruminant Meat and Fats

Devatkal Suresh*, Kalpana S., Reddy P. Baswa

ICAR-National Research Centre on Meat, Chengicherla, Hyderabad, Telangana, India, 500092

*Corresponding author Email address: Suresh.Devatkal@icar.gov.in

Online published on 19 December, 2019.

Abstract

Conjugated linoleic acid (CLA) is a naturally occurring bioactive fatty acid in meat and fats of ruminant animals. CLA is gaining much attention due to its nutritional and therapeutic properties. In this study, the presence of CLA in fat and muscle tissues of sheep, goat, and buffalo was established. The CLA content of a large number of fat samples (N=140) was analyzed by measuring the UV-VIS absorbance at 233 nm and comparing with the standard CLA absorbance curve. GC/FID and GC/MS were used for further used for quantification and confirmation. Results showed a CLA concentration of 2.5 to 8.5 mg/g fat and 1.4 to 3.7 mg/g of meat in different ruminant species. Further, rendered fat had a significantly (P<0.05) higher CLA than meat and fat samples. Gas chromatography and mass-spectrometry studies confirmed the presence of CLA in the samples analyzed.

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Keywords

Conjugated linoleic acid, Ruminant meat, GC-MS, Bioactive compound, Rendered fat.

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