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Journal of Camel Practice and Research
Year : 2019, Volume : 26, Issue : 3
First page : ( 255) Last page : ( 258)
Print ISSN : 0971-6777. Online ISSN : 2277-8934.
Article DOI : 10.5958/2277-8934.2019.00040.7

Assessment of genetic variability in Kappa casein gene in Indian dromedary

Yamini, Gahlot G.C., Pannu Urmila, Ashraf Mohammed, Choudhary Sanjay1

Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, RAJUVAS, Bikaner, 334001, Rajasthan

1Livestock Production Management Division, ICAR-National Dairy Research Institute, Karnal, Haryana

email: rajsaya07@gmail.com

Online published on 30 January, 2020.

Abstract

The present study was undertaken to explore the genetic variability in kappa casein gene in Bikaneri camel. DNA from camel were extracted through spin column method. PCR was carried out in a final reaction volume of 50 μl and the reaction mixture was subjected to standard PCR protocol. PCR products (488 bp) were digested with AluI. The RFLP analysis of kappa casein gene through AluI restriction enzyme detected 2 genotypic pattern in all the studied animals in a genotypic frequency of 0.41 and 0.59 for CT and TT genotype and gene frequency 0.79 (T allele) and 0.21 (C allele). In conclusion, the genetic polymorphism in kappa casein gene which is detected first time in Indian camel is considered the best way for enhancing milk composition by selection of animals with superior traits.

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Keywords

Genetic polymorphism, Kappa casein, Polymerase chain reaction.

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