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Journal of Animal Research
Year : 2015, Volume : 5, Issue : 4
First page : ( 767) Last page : ( 772)
Print ISSN : 2249-5290. Online ISSN : 2277-940X.
Article DOI : 10.5958/2277-940X.2015.00127.8

Assessment of Antimicrobial Activity of Pomegranate Leaf Extract against Bacterial Isolates of Otitis Externa and Diarrhea of Dogs

Tomar Ramkumar Singh, Gupta Amit Kumar, Sannat Chandrahas*, Agrawal Ritu, Hirpurkar S.D.

Department of Veterinary Microbiology, College of Veterinary Science and A.H., Anjora, Durg, Chhattisgarh, India

*Corresponding author: C Sannat; Email: csannat@rediffmail.com

Online published on 29 January, 2016.


Bacterial pathogens were isolated from cases of otitis externa and diarrhoea in dogs at Durg and Raipur districts of Chhattisgarh. All isolates were further subjected for antibiogram profile using commonly used antibiotics. Total 105 bacterial isolates were obtained which included Staphylococcus sp. and E. coli as predominant pathogens followed by Proteus, Klebsiella, Pseudomonas, Micrococcus and Salmonella. In cases of otitis externa, ciprofloxacin was the most effective antibiotic (67.27%) followed by Gentamicin (61.81%), Cephalaxin (40%), Chloramphenicol (32.72%), Amoxycillin (21.81%), Ampicillin (20%), Doxycycline (16.36%), Penicillin (12.72%) and Erythromycin (3.63%). However, Chloromphenical (72%) was reported as the most effective drug against bacterial isolates of diarrhoea followed by Ciprofloxacin (70%), Gentamicin (52%), Cephalaxin (40%), Doxycyline (30%), Ampicillin (14%), Amoxycillin (6%) and Erythromycin (2%). Cold extract of pomegranate leaves was then assessed for antibacterial activity against all isolates by disc diffusion and tube dilution methods. The zones of inhibition of pomegranate leaves extract (PLE) against Staphylococcus aureus, Pseudomonas aeruginosa, Proteus, Klebsiella, E. coli and Salmonella were 18.8 ± 0.050, 5±0.70, 7.8±0.73, 16.2±0.86, 15.6±0.92 and 15.2±0.86 mm respectively. The zone of inhibition with Staphylococcus was significantly higher (P<0.05) than other bacteria. The minimum bactericidal concentration against Staphylococcus aureus, Klebsiella, E. coli and Salmonella were 33.33±8.34, 66.66±16.68, 66.66±16.68 and 33.33±8.34 mg/ml respectively. Staphylococcus aureus showed maximum sensitivity to pomegranate leaf extract amongst all the organisms studied, followed by Klebsiella, E. coli and Salmonella. Although, Proteus and Pseudomonas were refractory to PLE, but moderate sensitivity was noticed even against few multidrug resistant isolates.



Pomegranate leaf extract, bacteria, otitis externa, diarrhoea, dog.


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