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Indian Journal of Scientific Research
Year : 2014, Volume : 5, Issue : 1
First page : ( 139) Last page : ( 152)
Print ISSN : 0976-2876. Online ISSN : 2250-0138.

Biodegradation study of reactive blue 172 by Shewanella haliotis DW01 isolated from lake sediment

Birmole Radhika, Patade Swamini, Sirwaiya Vishwas, Bargir Firdaus, Aruna K1

Department of Microbiology, Wilson College, Mumbai, India

1Corresponding author

Online published on 8 April, 2015.


Reactive Blue 172 (RB-172) dyeis a reactive sulphonateddiazo dye commercially used in the textile industry. Anovel bacterialstrain which degrades RB-172 was isolated from lake watersediment and identified as Shewanella haliotis DW01 based on physiological and biochemical tests together with 16S rRN Agene sequence analysis. The 93% dye decolorizing activity of 50 ppm RB-172 by Shewanella haliotis DW01 was obtained with an inoculum size of 0.8 O.D530nm inoptimized Luria Bertani medium (Tryptone 0.5%, yeast extract 0.5%, NaCl 1%) pH9.5 at 357°C kept under static condition for 12h with high COD reduction. The isolate demonstrated significant decolorization over a wide range of NaCl concentration (1–7%). The addition of inorganic nitrogenous compounds to the medium significantly decreased the decolorization process. Lactate and pyruvate were supporting RB-172 decolorization. Maximum dye concentration decolorized by the isolate after 12h was 550ppm. An improved decolorization performance was obtained in the presence of higher biomass concentration. Decolorization assay using pre-grown cell mass of Shewanella haliotis DW01 exhibited 87% decolorization after 6h. The bacterium was immobilized and the decolorization process was monitored using UV-Vis Spectrophotometry. The degradation of dye was confirmed by HPTL Cand HPL Canalysis. Toxicity results suggested that degradation products of RB-172 werenontoxic to the common crop such as Phaseolus mungo and Triticum aestivum. It also showed ability to decolorize seven different dyes and mixtures of few dyes.



Shewanella haliotis DW01, Reactive Blue 172 (RB-172), decolorization, biodegradation.


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